2POL
THREE-DIMENSIONAL STRUCTURE OF THE BETA SUBUNIT OF ESCHERICHIA COLI DNA POLYMERASE III HOLOENZYME: A SLIDING DNA CLAMP
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.610, 68.350, 82.350 |
| Unit cell angles | 90.00, 114.26, 90.00 |
Refinement procedure
| Resolution | ? - 2.500 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 3.450 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.400 * |
| Rmerge | 0.073 * |
| Total number of observations | 101590 * |
| Number of reflections | 30957 * |
| Completeness [%] | 94.4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG400 | 30 (%) | |
| 2 | 1 | reservoir | 100 (mM) | ||
| 3 | 1 | reservoir | MES | 100 (mM) |
