2POH

Structure of Phage P22 Tail Needle gp26

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	40.398, 114.029, 171.917
Unit cell angles	90.00, 90.74, 90.00

Refinement procedure

Resolution	? - 2.100
R-free	0.23200
Structure solution method	AB INITIO
RMSD bond length	0.007
RMSD bond angle	0.023
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.210
High resolution limit [Å]	2.100	2.100
Rmerge		0.353
Number of reflections	90017
<I/σ(I)>	15.9	4.6
Completeness [%]	99.2	98.7
Redundancy	5.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	8% PEG8000; 0.1M Ammonium Sulfate, 0.05M NaCl, 0.1M Sodium Acetate pH4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	8% PEG8000; 0.1M Ammonium Sulfate, 0.05M NaCl, 0.1M Sodium Acetate pH4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	8% PEG8000; 0.1M Ammonium Sulfate, 0.05M NaCl, 0.1M Sodium Acetate pH4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K