2POF
Crystal structure of CDP-diacylglycerol pyrophosphatase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-26 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.246, 65.418, 80.926 |
| Unit cell angles | 90.00, 90.21, 90.00 |
Refinement procedure
| Resolution | 25.440 - 1.400 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.25700 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.073 | 0.293 |
| Number of reflections | 68386 | |
| <I/σ(I)> | 16.4 | |
| Completeness [%] | 89.4 | 49.5 |
| Redundancy | 7 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 2.1 M Malic Acid, 50% PEG 400, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
