2POD
Crystal structure of a member of enolase superfamily from Burkholderia pseudomallei K96243
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-09 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97960 |
| Spacegroup name | I 4 |
| Unit cell lengths | 146.771, 146.771, 85.531 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.340 |
| R-factor | 0.2114 |
| Rwork | 0.209 |
| R-free | 0.27451 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.272 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.3.0034) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.420 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Rmerge | 0.090 | 0.600 |
| Number of reflections | 38379 | |
| <I/σ(I)> | 5.2 | 0.9 |
| Completeness [%] | 88.8 | 86.2 |
| Redundancy | 3.7 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 294 | 100mM Bis-Tris-HCl, pH 5.5, 45% MPD, 200mM Ammonium acetate, 10% Glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
