2PO4
X-ray crystal structure of polymerase domain of the bacteriophage N4 virion RNA polymerase
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-30 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1, 0.9790, 0.9793, 0.9649 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.047, 103.258, 159.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.000 |
Rwork | 0.219 |
R-free | 0.24800 |
Structure solution method | MAD |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.071 | 0.596 |
Number of reflections | 91553 | |
<I/σ(I)> | 10.1 | |
Completeness [%] | 98.3 | 98.9 |
Redundancy | 3.9 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 300 | 10mM Tris-HCl (pH 8), 100mM NaCl and 0.1mM EDTA , pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 300 | 10mM Tris-HCl (pH 8), 100mM NaCl and 0.1mM EDTA , pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 300 | 10mM Tris-HCl (pH 8), 100mM NaCl and 0.1mM EDTA , pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |