2PO2
Crystal structure of the P. abyssi exosome RNase PH ring complexed with CDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE D03B-MX1 |
Synchrotron site | LNLS |
Beamline | D03B-MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.427 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 93.498, 93.498, 125.714 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.800 - 2.410 |
R-factor | 0.18603 |
Rwork | 0.182 |
R-free | 0.25852 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pnz |
RMSD bond length | 0.021 |
RMSD bond angle | 2.106 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.630 | 2.540 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 25053 | |
<I/σ(I)> | 11.1 | 2.8 |
Completeness [%] | 99.2 | 96 |
Redundancy | 5.5 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1 M Bis-Tris, 45% MPD and 0.1 M LiCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |