2PO2
Crystal structure of the P. abyssi exosome RNase PH ring complexed with CDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.427 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 93.498, 93.498, 125.714 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.800 - 2.410 |
| R-factor | 0.18603 |
| Rwork | 0.182 |
| R-free | 0.25852 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pnz |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.106 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.630 | 2.540 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 25053 | |
| <I/σ(I)> | 11.1 | 2.8 |
| Completeness [%] | 99.2 | 96 |
| Redundancy | 5.5 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 0.1 M Bis-Tris, 45% MPD and 0.1 M LiCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291.0K |
