2PND
Structure or murine CRIg
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-06 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.334, 50.684, 62.809 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.530 - 1.000 |
| R-factor | 0.12305 |
| Rwork | 0.122 |
| R-free | 0.14160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2icc |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.535 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.000 |
| High resolution limit [Å] | 0.970 | 0.970 |
| Number of reflections | 51825 | |
| <I/σ(I)> | 15.7 | 2.7 |
| Completeness [%] | 86.3 | 24.9 |
| Redundancy | 6.2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 298 | 100 mM NaCl, 25 mM TRIS, 20 % PEG 3350, 0.2 M Potassium tartrate, pH 7.5, EVAPORATION, temperature 298K |
