2PN4
Crystal Structure of Hepatitis C Virus IRES Subdomain IIa
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 8-BM |
Synchrotron site | APS |
Beamline | 8-BM |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2006-03-04 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.286, 48.730, 119.999 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 59.980 - 2.320 |
R-factor | 0.26405 |
Rwork | 0.261 |
R-free | 0.32037 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pn3 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.835 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 59.980 | 2.430 |
High resolution limit [Å] | 2.320 | 2.320 |
Rmerge | 0.108 | 0.714 |
Number of reflections | 12228 | |
<I/σ(I)> | 17.5 | 2.4 |
Completeness [%] | 95.9 | 100 |
Redundancy | 5.5 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.2 | 290 | 30% MPD, 80mM SrCl2, 25mM NaCl, 50mM Cacodylate equilibrated with 60%MPD, pH 6.2, EVAPORATION, temperature 290.0K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | 30% MPD | ||
2 | 1 | 1 | SrCl2 | ||
3 | 1 | 1 | NaCl | ||
4 | 1 | 1 | Cacodylate | ||
5 | 1 | 2 | 60%MPD |