2PN3
Crystal Structure of Hepatitis C Virus IRES Subdomain IIa
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2004-10-30 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9203, 0.9197, 0.90686 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 48.945, 48.945, 120.942 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 - 2.900 |
R-factor | 0.22969 |
Rwork | 0.229 |
R-free | 0.28263 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.885 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.081 | 0.345 |
Number of reflections | 3420 | |
<I/σ(I)> | 27.5 | 4.6 |
Completeness [%] | 93.7 | 75.1 |
Redundancy | 11.2 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6.2 | 290 | 5% MPD, 5mM MgSO4, 12.5mM NaCl, 50mM KCl, 25mM Cacodylate equilibrated with 65%MPD, pH 6.2, EVAPORATION, temperature 290.0K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | 5% MPD | ||
2 | 1 | 1 | MgSO4 | ||
3 | 1 | 1 | NaCl | ||
4 | 1 | 1 | KCl | ||
5 | 1 | 1 | Cacodylate | ||
6 | 1 | 2 | 65% MPD |