2PN0
Prokaryotic transcription elongation factor GreA/GreB from Nitrosomonas europaea
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-12-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97920 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 76.628, 80.838, 171.387 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.900 - 1.700 |
R-factor | 0.1781 |
Rwork | 0.177 |
R-free | 0.20550 |
Structure solution method | SAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.580 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.900 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.068 | 0.609 |
Number of reflections | 58450 | |
<I/σ(I)> | 37.2 | 1.76 |
Completeness [%] | 99.3 | 90.2 |
Redundancy | 7.6 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 0.05 M Calcium chloride, 0.1 M Na Acetate, 20% PEG3350, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |