2PN0
Prokaryotic transcription elongation factor GreA/GreB from Nitrosomonas europaea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97920 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 76.628, 80.838, 171.387 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.900 - 1.700 |
| R-factor | 0.1781 |
| Rwork | 0.177 |
| R-free | 0.20550 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.580 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.900 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.068 | 0.609 |
| Number of reflections | 58450 | |
| <I/σ(I)> | 37.2 | 1.76 |
| Completeness [%] | 99.3 | 90.2 |
| Redundancy | 7.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 291 | 0.05 M Calcium chloride, 0.1 M Na Acetate, 20% PEG3350, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
