2PMV
Crystal Structure of Human Intrinsic Factor- Cobalamin Complex at 2.6 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.6059 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.100, 67.300, 147.700 |
| Unit cell angles | 90.00, 96.80, 90.00 |
Refinement procedure
| Resolution | 37.530 - 2.600 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.24900 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.900 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.070 | 0.491 |
| Number of reflections | 55707 | |
| <I/σ(I)> | 16.5 | 2.2 |
| Completeness [%] | 91.1 | 54.7 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 295 | 10% PEG20000, 100mM MES, 20mM CaCL2 and 9mM BaCL2, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
