2PMS
Crystal structure of the complex of human lactoferrin N-lobe and lactoferrin-binding domain of pneumococcal surface protein A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-14 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 32 |
| Unit cell lengths | 130.180, 130.180, 80.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.910 |
| R-factor | 0.20541 |
| Rwork | 0.203 |
| R-free | 0.24906 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h45 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.167 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.000 |
| High resolution limit [Å] | 2.910 | 2.910 |
| Rmerge | 0.098 | 0.359 |
| Number of reflections | 33481 | |
| <I/σ(I)> | 7 | 3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 2.9 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 30% (v/v) PEG 400, 0.2 M Lithium Sulfate, 0.1 M Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
