2PMR
Crystal structure of a protein of unknown function from Methanobacterium thermoautotrophicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-14 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97958 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 37.370, 94.936, 46.258 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.320 |
| R-factor | 0.17545 |
| Rwork | 0.175 |
| R-free | 0.19130 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.030 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXCD |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.468 | 1.390 |
| High resolution limit [Å] | 1.320 | 1.320 |
| Rmerge | 0.088 | 0.439 |
| Number of reflections | 19764 | |
| <I/σ(I)> | 14.8 | 3.8 |
| Completeness [%] | 98.7 | 92.4 |
| Redundancy | 7.7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 294 | 55% MPD, pH 7.0, VAPOR DIFFUSION, temperature 294K |
