2PM6
Crystal Structure of yeast Sec13/31 edge element of the COPII vesicular coat, native version
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 200 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-06-01 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 128.387, 52.343, 133.128 |
| Unit cell angles | 90.00, 108.62, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.450 |
| R-factor | 0.254 |
| Rwork | 0.251 |
| R-free | 0.30100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | From SAD/MAD-determined structure |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.319 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.540 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.041 | 0.350 |
| Number of reflections | 56172 | |
| <I/σ(I)> | 20 | 2.37 |
| Completeness [%] | 94.7 | 93.2 |
| Redundancy | 2.4 | 2.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 15% PEG 4000, 10% DMSO, 6% dioxanie, 0.1M Tris-HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
