2PM5
Human alpha-defensin 1 derivative (HNP1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-04 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 42 2 2 |
| Unit cell lengths | 57.409, 57.409, 96.967 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.20106 |
| Rwork | 0.200 |
| R-free | 0.21789 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dfn |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.740 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.060 | 0.518 |
| Number of reflections | 6220 | |
| <I/σ(I)> | 31.2 | 2.1 |
| Completeness [%] | 91.1 | 53.8 |
| Redundancy | 9.4 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 0.1 M sodium citrate tribasic dihydrate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
