2PLW
Crystal structure of a ribosomal RNA methyltransferase, putative, from Plasmodium falciparum (PF13_0052).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-11 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91770 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.595, 59.798, 71.295 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.830 - 1.700 |
| R-factor | 0.205 |
| Rwork | 0.202 |
| R-free | 0.24700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2nyu |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.522 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.073 | 0.324 |
| Number of reflections | 20472 | |
| <I/σ(I)> | 9.9 | 3.7 |
| Completeness [%] | 98.5 | 87.9 |
| Redundancy | 6.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 298 | 24% PEG 3350, 0.1 M (NH4)2SO4, 0.1 M Bis-Tris pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
