2PLT

STRUCTURE DETERMINATION OF PLASTOCYANIN FROM A CRYSTAL SPECIMEN WITH HEMIHEDRAL TWINNING FRACTION OF ONE-HALF

Experimental procedure

Spacegroup name	P 32
Unit cell lengths	61.800, 61.800, 25.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	? - 1.500
R-factor	0.168
Rwork	0.168
RMSD bond length	0.020
RMSD bond angle	1.880
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	1.500 *
Rmerge	0.041 *
Total number of observations	46606 *
Number of reflections	15938 *
Completeness [%]	92.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.6 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.7 (M)
2	1	reservoir		0.1 (M)
3	1	reservoir		0.1 (M)
4	1	reservoir	sodium phosphate	0.08 (M)
5	1	drop	protein	12.6 (M)