2PLS
Structural Genomics, the crystal structure of the CorC/HlyC transporter associated domain of a CBS domain protein from Chlorobium tepidum TLS
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97921, 0.97938 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 108.243, 108.493, 106.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.510 - 2.150 |
| R-factor | 0.1787 |
| Rwork | 0.176 |
| R-free | 0.23203 |
| Structure solution method | MAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.646 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.510 | 2.230 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.101 | 0.513 |
| Number of reflections | 68297 | |
| <I/σ(I)> | 30.4 | 3.3 |
| Completeness [%] | 99.1 | 97 |
| Redundancy | 8.2 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2M MgCl, 0.1M Tris-HCl, 25% PEG3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
