2PLO
D-(GTATACC) low temperature (100K)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.962 |
| Spacegroup name | P 61 |
| Unit cell lengths | 44.783, 44.783, 41.393 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.400 |
| R-factor | 0.1773 |
| Rwork | 0.170 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2pl8 |
| RMSD bond length | 0.098 |
| RMSD bond angle | 0.295 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.500 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.051 | 0.191 |
| Number of reflections | 9382 | |
| <I/σ(I)> | 20.2 | 3.9 |
| Completeness [%] | 94.5 | 99.8 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 20 mg dissolved in 0.2 ml of 15 % MPD, 10-2 M sodium cacodylate buffer. Additives : 10-5M sodium azide, 10-2 M MgCl2 and 2.10-2 M spermine chloride, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 10 | 1 | 2 | spermine chloride | ||
| 2 | 1 | 1 | sodium cacodylate | ||
| 3 | 1 | 1 | sodium azide | ||
| 4 | 1 | 1 | MgCl2 | ||
| 5 | 1 | 1 | spermine chloride | ||
| 6 | 1 | 2 | MPD | ||
| 7 | 1 | 2 | sodium cacodylate | ||
| 8 | 1 | 2 | sodium azide | ||
| 9 | 1 | 2 | MgCl2 |
