2PLN
Crystal structure analysis of HP1043, an orphan resonse regulator of h. pylori
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 6B |
Synchrotron site | PAL/PLS |
Beamline | 6B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-05-15 |
Detector | BRUKER PROTEUM 300 |
Wavelength(s) | 0.97940, 0.97952, 0.97962, 0.98742 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 88.967, 41.311, 31.689 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.500 - 1.800 |
R-factor | 0.208 |
Rwork | 0.206 |
R-free | 0.24000 |
Structure solution method | MAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.461 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 11373 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 7.3 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 8K 3%, 100MM MES, 200MM CA-CACODYLATE, pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K |