2PLJ
Crystal structure of lysine/ornithine decarboxylase complexed with putrescine from Vibrio vulnificus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-15 |
| Detector | SBC-3 |
| Wavelength(s) | 0.979269 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 82.074, 88.683, 111.843 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.720 - 1.700 |
| R-factor | 0.1824 |
| Rwork | 0.181 |
| R-free | 0.21311 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1f3t |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.419 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.074 | 0.633 |
| Number of reflections | 90404 | |
| <I/σ(I)> | 35.9 | 2.46 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 277 | 35% PEG-4000, 0.1M Tris HCl pH8.4, 0.2M Magnesium chloride, 0.5% w/v n-octyl-b-D-glucoside , VAPOR DIFFUSION, HANGING DROP, temperature 277K |
