2PLH

STRUCTURE OF ALPHA-1-PUROTHIONIN AT ROOM TEMPERATURE AND 2.8 ANGSTROMS RESOLUTION

Experimental procedure

Collection date	1989-01-01
Detector	CRAD, P21
Wavelength(s)	1.5418
Spacegroup name	I 4 2 2
Unit cell lengths	53.590, 53.590, 69.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.155
RMSD bond length	0.012
RMSD bond angle	0.026
Data reduction software	DTP
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000
High resolution limit [Å]	2.500
Rmerge	0.123
Number of reflections	2106
Completeness [%]	61.5
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	180 (mg/ml)
2	1	drop	sodium cacodylate	75 (mM)
3	1	drop	sec-butanol	15 (%(v/v))
4	1	reservoir	sec-butanol	8 (%(v/v))
5	1	reservoir	sodium cacodylate	90 (mM)
6	1	reservoir		20 (%(v/v)sat)