2PLG
Crystal structure of T110839 protein from Synechococcus elongatus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.98000 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 98.185, 98.185, 144.679 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.540 - 2.600 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.550 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.100 | 0.630 |
| Number of reflections | 26434 | |
| <I/σ(I)> | 10 | |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 27.6 | 18.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 1.0 M Lithium sulfate, 0.5 M Ammonium sulfate, 0.1 M Tri-Na citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
