2PLC

PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C FROM LISTERIA MONOCYTOGENES

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1996-06-12
Detector	SIEMENS
Spacegroup name	P 31 2 1
Unit cell lengths	82.500, 82.500, 92.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.198
Rwork	0.198
R-free	0.24900
Structure solution method	MOLECULAR REPLACEMENT AND SIG
Starting model (for MR)	1ptd
RMSD bond length	0.006
RMSD bond angle	24.400 *
Data reduction software	XDS
Data scaling software	CCP4 ((SCALA))
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.000	1.950
High resolution limit [Å]	1.900	1.900
Rmerge	0.069 *	0.250
Total number of observations	79316 *
Number of reflections	25523
<I/σ(I)>	8.9	3.6
Completeness [%]	88.5	57.6
Redundancy	3.1	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	4 *	PROTEIN WAS CRYSTALLIZED FROM 0.1 M NA-HEPES PH 7.5, 0.8 M NA-K-TARTRATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6.5 (mg/ml)
2	1	reservoir	Na-Hepes	0.1 (M)
3	1	reservoir	Na/K tartrate	0.8 (M)
4	1	drop	precipitant