2PLB
D(GTATACC) under hydrostatic pressure of 1.39 GPa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID27 |
| Synchrotron site | ESRF |
| Beamline | ID27 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.3738 |
| Spacegroup name | P 61 |
| Unit cell lengths | 42.830, 42.830, 40.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.1866 |
| Rwork | 0.181 |
| R-free | 0.22200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2pl8 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 0.030 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.650 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.047 | 0.331 |
| Number of reflections | 5518 | |
| <I/σ(I)> | 11.4 | 2.2 |
| Completeness [%] | 98.5 | 99 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 20 mg of DNA dissolved in 0.2 ml of 15% MPD solution cacodylate buffer 10-2 M. Additives: 10-5 M sodium azide, 10-2 M MgCl2, 2.10-2 M spermine chloride. Reservoir: same solution but 50 % MPD, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | 15% MPD | ||
| 10 | 1 | 2 | spermine chloride | ||
| 2 | 1 | 1 | cacodylate buffer | ||
| 3 | 1 | 1 | sodium azide | ||
| 4 | 1 | 1 | MgCl2 | ||
| 5 | 1 | 1 | spermine chloride | ||
| 6 | 1 | 2 | 50% MPD | ||
| 7 | 1 | 2 | cacodylate buffer | ||
| 8 | 1 | 2 | sodium azide | ||
| 9 | 1 | 2 | MgCl2 |
