2PL7
Orhorhombic crystal structure of hydrophobin HFBII in the presence of a detergent
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X12 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X12 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 25.300, 57.100, 72.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.000 |
| R-factor | 0.1796 |
| R-free | 0.15150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r2m |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.034 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | SHELX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.200 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.101 | 0.242 |
| Number of reflections | 107245 | |
| <I/σ(I)> | 8.82 | 3.9 |
| Completeness [%] | 98.3 | 96.9 |
| Redundancy | 3.4 | 2.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.91 | 293 | 0.1 M lithium sulphate, 20% polyethylene glycol (MW2000), 0.1M Tris (pH 8.5), pH 6.91, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
