2PL6
Monoclinic crystal structure of hydrophobin HFBII in presence of a detergent
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2006-07-08 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.080, 66.410, 79.770 |
| Unit cell angles | 90.00, 99.23, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| Rwork | 0.224 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1r2m |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.306 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.500 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.119 | 0.472 |
| Number of reflections | 61949 | |
| <I/σ(I)> | 7.7 | 3.1 |
| Completeness [%] | 98.6 | 98.4 |
| Redundancy | 2.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.48 | 293 | 0.1 M lithium sulphate, 20% polyethylene glycol (MW 2000), 0.1 M tris (pH 8.5), pH 8.48, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
