2PL4
D-(GGTATACC) under 0.55 GPa hydrostatic pressure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID27 |
| Synchrotron site | ESRF |
| Beamline | ID27 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-11-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.3738 |
| Spacegroup name | P 61 |
| Unit cell lengths | 43.710, 43.710, 40.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 1.650 |
| R-factor | 0.1693 |
| Rwork | 0.168 |
| R-free | 0.20100 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2pkv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.028 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 1.750 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.043 | 0.188 |
| Number of reflections | 5057 | |
| <I/σ(I)> | 10.2 | 3.6 |
| Completeness [%] | 94.1 | 86.2 |
| Redundancy | 3.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 10 mg of octamer dissolved in cacodylate buffer (10-2 M, 15 % MPD) additives : 10-5 M sodium azide, 10-2 M MgCl2, 2.10-2 M spermine chloride. Reservoir: same solution but 50 % MPD, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | cacodylate buffer | ||
| 10 | 1 | 2 | spermine chloride | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | sodium azide | ||
| 4 | 1 | 1 | MgCl2 | ||
| 5 | 1 | 1 | spermine chloride | ||
| 6 | 1 | 2 | cacodylate buffer | ||
| 7 | 1 | 2 | MPD | ||
| 8 | 1 | 2 | sodium azide | ||
| 9 | 1 | 2 | MgCl2 |
