2PL3
Human DEAD-box RNA helicase DDX10, DEAD domain in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-22 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.04123 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 63.500, 63.500, 304.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.300 - 2.150 |
| R-factor | 0.21156 |
| Rwork | 0.210 |
| R-free | 0.24757 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gxs |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.695 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.3.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.300 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.048 | 0.160 |
| Number of reflections | 20889 | |
| <I/σ(I)> | 54.1 | 24.5 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 25.4 | 26.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 20% Ethanol, 50mM Sodium chloride, 100mM Tris-HCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
