Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-20 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.07217 |
Spacegroup name | P 65 |
Unit cell lengths | 102.645, 102.645, 62.641 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.540 |
R-factor | 0.21029 |
Rwork | 0.206 |
R-free | 0.25284 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | activated ecoli PhoP receiver domain structure without alpha helix 4 |
RMSD bond length | 0.019 |
RMSD bond angle | 1.826 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.540 |
High resolution limit [Å] | 2.450 | 2.450 |
Number of reflections | 13785 | |
<I/σ(I)> | 13.51 | 1.36 |
Completeness [%] | 98.8 | 99.1 |
Redundancy | 4.96 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 298 | precipitant: Sodium thiocyanate, Peg3350, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 298K |