2PKW
Crystal structure of UPF0341 protein yhiQ from Salmonella typhimurium, Northeast Structural Genomics Consortium Target StR221
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-13 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97880 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 41.967, 70.248, 77.788 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.530 - 2.100 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.25900 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.530 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.068 | 0.329 |
Number of reflections | 25710 | |
<I/σ(I)> | 28.1 | 4 |
Completeness [%] | 99.6 | 99.5 |
Redundancy | 6.6 | 6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH UNDER OIL | 8 | 291 | 2 micro-L of protein solution containing 10 mM Tris-HCl pH 7.5, 100 mM Sodium chloride, 5 mM DTT was mixed with equal volume of 100 mM Tris-HCl pH 8.0, 40% PEG 1000, and 100 mM Magnesium acetate, MICROBATCH UNDER OIL, temperature 291K |