2PKV
D-(GGTATACC) ambient pressure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID27 |
Synchrotron site | ESRF |
Beamline | ID27 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Collection date | 2006-11-10 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.3738 |
Spacegroup name | P 61 |
Unit cell lengths | 45.030, 45.030, 41.750 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 1.600 |
R-factor | 0.151 |
Rwork | 0.149 |
R-free | 0.18910 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 115D FROM PDB |
RMSD bond length | 0.011 |
RMSD bond angle | 0.029 |
Data reduction software | XDS |
Data scaling software | SCALA |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.045 | 0.330 |
Number of reflections | 6228 | |
<I/σ(I)> | 9.3 | 2.3 |
Completeness [%] | 94.5 | 96.2 |
Redundancy | 4 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 290 | 10 mg of lyophilized DNA dissolved in 0.2 ml of a 15 % MPD solution with additives: 10-5 M sodium azide, 10-2 M MgCl2 and 2.10-2 spermine chloride. Reservoir : same solution but 50 % MPD, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
2 | 1 | 1 | sodium azide | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 1 | spermine chloride | ||
5 | 1 | 2 | MPD | ||
6 | 1 | 2 | sodium azide | ||
7 | 1 | 2 | MgCl2 | ||
8 | 1 | 2 | spermine chloride |