2PKH
Structural Genomics, the crystal structure of the C-terminal domain of histidine utilization repressor from Pseudomonas syringae pv. tomato str. DC3000
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97925 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.845, 100.955, 130.187 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.870 - 1.950 |
| R-factor | 0.22243 |
| Rwork | 0.219 |
| R-free | 0.27728 |
| Structure solution method | SAD |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.014 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.870 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.095 | 0.577 |
| Number of reflections | 97149 | |
| <I/σ(I)> | 24.53 | 2.83 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 5.6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 100mM HEPES, 200mM Ammonium sulfate, 10% Isopropanol, 16% PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
