2PKD

Crystal structure of CD84: Insite into SLAM family function

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9A
Synchrotron site	NSLS
Beamline	X9A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-26
Detector	MAR CCD 165 mm
Wavelength(s)	0.961, 0.9797, 0.9794
Spacegroup name	C 2 2 21
Unit cell lengths	61.013, 170.982, 148.527
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.043
R-factor	0.201
Rwork	0.197
R-free	0.27700
Structure solution method	MAD
RMSD bond length	0.023
RMSD bond angle	1.938
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.050
High resolution limit [Å]	1.980	4.260	1.980
Rmerge	0.072	0.058	0.406
Number of reflections	39739
<I/σ(I)>	11.3
Completeness [%]	90.2	99.3	15.1
Redundancy	3.6	3.6	1.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	20% PEG3350, 0.3 M MgCl2, 0.1 M, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	20% PEG3350, 0.3 M MgCl2, 0.1 M, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K
1	VAPOR DIFFUSION, SITTING DROP	7.5	294	20% PEG3350, 0.3 M MgCl2, 0.1 M, HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K