2PKA

REFINED 2 ANGSTROMS X-RAY CRYSTAL STRUCTURE OF PORCINE PANCREATIC KALLIKREIN A, A SPECIFIC TRYPSIN-LIKE SERINE PROTEINASE. CRYSTALLIZATION, STRUCTURE DETERMINATION, CRYSTALLOGRAPHIC REFINEMENT, STRUCTURE AND ITS COMPARISON WITH BOVINE TRYPSIN

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	90.200, 90.200, 159.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.050
R-factor	0.22 *
Rwork	0.220
Refinement software	EREF

Data quality characteristics

	Overall
High resolution limit [Å]	2.050 *
Rmerge	0.102 *
Total number of observations	198249 *
Number of reflections	35517 *
Completeness [%]	86.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	1 (M)
2	1	drop	phosphate	0.1 (M)
3	1	drop	benzamidine	10 (mM)
4	1	drop	protein	60 (mg/ml)