2PK7
Crystal structure of the Q4KFT4_PSEF5 protein from Pseudomonas fluorescens. NESG target PlR1
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12C |
Synchrotron site | NSLS |
Beamline | X12C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-03 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.97907, 0.97940, 0.96791 |
Spacegroup name | P 1 |
Unit cell lengths | 29.415, 34.363, 35.832 |
Unit cell angles | 76.39, 76.12, 73.58 |
Refinement procedure
Resolution | 25.990 - 2.200 |
R-factor | 0.223 |
Rwork | 0.223 |
R-free | 0.24600 |
Structure solution method | MAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.031 | 0.062 |
Number of reflections | 11707 | |
<I/σ(I)> | 25.5 | 12.6 |
Completeness [%] | 88.4 | 83.9 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 24-28% PEG 3350, 0.2M Sodium chloride, 0.1M Bis-Tris, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |