2PJZ
The crystal structure of putative Cobalt transport ATP-binding protein (cbiO-2), ST1066
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-17 |
| Detector | RIGAKU JUPITER 210 |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 102.094, 102.094, 104.662 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.800 - 1.900 |
| R-factor | 0.19821 |
| Rwork | 0.196 |
| R-free | 0.24172 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.549 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 25918 | |
| <I/σ(I)> | 53.7 | 10.12 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.6 | 21.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.5M Ammonium sulfate, 0.1M Sodium citrate tribasic dihydrate, pH5.6, 1.0M Lithium sulfate monohydrate, pH8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
