2PJW
The Vps27/Hse1 complex is a GAT domain-based scaffold for ubiquitin-dependent sorting
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2006-07-12 |
Wavelength(s) | 0.979493, 0.972425 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 62.400, 62.400, 94.560 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.920 - 3.010 |
R-factor | 0.222 |
Rwork | 0.222 |
R-free | 0.28500 |
Structure solution method | TWO WAVELENGTH MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | HKL-2000 |
Phasing software | SnB |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.920 | 3.140 |
High resolution limit [Å] | 3.010 | 3.010 |
Number of reflections | 7506 | |
<I/σ(I)> | 18.1 | |
Redundancy | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.85 | 288 | 1.1-1.4 M ammonium sulfate, 0.1 M Tris-HCl. Then micro-seeding into 1.25 M ammonium sulfate, 0.1 M Tris-HCl, pH 8.85, VAPOR DIFFUSION, SITTING DROP, temperature 288K |