2PJR
HELICASE PRODUCT COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 85.050, 62.600, 141.830 |
Unit cell angles | 90.00, 95.84, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.900 |
Rwork | 0.240 |
R-free | 0.29600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.700 |
Refinement software | CNS |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 |
High resolution limit [Å] | 2.900 |
Rmerge | 0.068 |
Number of reflections | 31850 |
Completeness [%] | 97.9 |
Redundancy | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 * | drop consists of 1:1 mixture of well and protein solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | DNA substrate | (mM) | |
10 | 1 | reservoir | 50 (mM) | ||
11 | 1 | reservoir | PEG4000 | 12-20 (%) | |
2 | 1 | drop | glucose | (%) | |
3 | 1 | drop | (mM) | ||
4 | 1 | drop | ADPNP | (mM) | |
5 | 1 | drop | dithiothreitol | (mM) | |
6 | 1 | drop | Tris-HCl | (mM) | |
7 | 1 | drop | EDTA | (mM) | |
8 | 1 | drop | 106 (mM) | ||
9 | 1 | reservoir | Tris-HCl | 100 (mM) |