2PIJ

Structure of the Cro protein from prophage Pfl 6 in Pseudomonas fluorescens Pf-5

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-07-21
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97935,0.97921,0.91165
Spacegroup name	P 31 2 1
Unit cell lengths	40.940, 40.940, 123.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.080 - 1.700
R-factor	0.221
Rwork	0.218
R-free	0.27100
Structure solution method	MAD
RMSD bond length	0.021
RMSD bond angle	1.855
Data reduction software	CrystalClear
Data scaling software	d*TREK (9.2SSI)
Phasing software	SOLVE (2.09)
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.080	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.055	0.434
Total number of observations		5236
Number of reflections	13741
<I/σ(I)>	13.5	3
Completeness [%]	98.1	90.7
Redundancy	4.84	4.28

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9.5	298	primary precipitating solution: 2.2M ammonium sulfate, 0.1M tris additional chemicals in drop: 2mM dithiothreitol, 0.2mM EDTA, 0.01% sodium azide, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	9.5	298	primary precipitating solution: 2.2M ammonium sulfate, 0.1M tris additional chemicals in drop: 2mM dithiothreitol, 0.2mM EDTA, 0.01% sodium azide, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	9.5	298	primary precipitating solution: 2.2M ammonium sulfate, 0.1M tris additional chemicals in drop: 2mM dithiothreitol, 0.2mM EDTA, 0.01% sodium azide, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K