2PIH
Crystal structure of Protein ymcA from Bacillus subtilis, NorthEast Structural Genomics target SR375
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 1 |
| Unit cell lengths | 29.322, 52.201, 55.448 |
| Unit cell angles | 61.85, 90.64, 90.01 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.207 |
| Rwork | 0.207 |
| R-free | 0.25400 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.869 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.053 | 0.142 |
| Number of reflections | 32962 | |
| <I/σ(I)> | 10.5 | 8.4 |
| Completeness [%] | 94.3 | 93.9 |
| Redundancy | 1.8 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100 mM NH4Cl, Tris-HCl, PEG3350, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
