2PIG
Crystal structure of ydcK from Salmonella cholerae at 2.38 A resolution. Northeast Structural Genomics target SCR6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-05-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97877 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 103.603, 103.603, 160.577 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.380 |
R-factor | 0.2195 |
Rwork | 0.220 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2f9c |
RMSD bond length | 0.005 |
RMSD bond angle | 1.299 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | COMO |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.380 |
High resolution limit [Å] | 2.300 | 4.930 | 2.300 |
Rmerge | 0.104 | 0.060 | 0.422 |
Number of reflections | 39556 | ||
<I/σ(I)> | 8.8 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 7.8 | 7.4 | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 10 mM MAGNESIUM SULFATE, 50 mM SODIUM CACODYLATE, 1.8 M LITHIUM SULFATE, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |