2PIE
Crystal structure of the FHA domain of RNF8 in complex with its optimal phosphopeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979462 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 34.632, 76.865, 120.744 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.350 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2csw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.539 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.400 |
| High resolution limit [Å] | 1.350 | 1.350 |
| Rmerge | 0.067 | 0.354 |
| Number of reflections | 35871 | |
| <I/σ(I)> | 12.2 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | batch crystallization | 8 | 277 | 4 mM Tris pH 8, 4 mM NaCl, 0.4 mM DTT, pH 8.0, batch crystallization, temperature 277K |
