2PI5
T7 RNA polymerase complexed with a phi10 promoter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 224.450, 73.791, 80.315 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.040 - 2.900 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.900 |
| Number of reflections | 29104 |
| <I/σ(I)> | 1.8 |
| Completeness [%] | 96.6 |
| Redundancy | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 300 | 50 mM Tris-HCl, 200 mM Li2SO4, 20% PEG 8000, 5% Glycerol, 15 mM magnesium acetate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Tris-HCl | ||
| 10 | 1 | 2 | magnesium acetate | ||
| 2 | 1 | 1 | Li2SO4 | ||
| 3 | 1 | 1 | PEG 8000 | ||
| 4 | 1 | 1 | Glycerol | ||
| 5 | 1 | 1 | magnesium acetate | ||
| 6 | 1 | 2 | Tris-HCl | ||
| 7 | 1 | 2 | Li2SO4 | ||
| 8 | 1 | 2 | PEG 8000 | ||
| 9 | 1 | 2 | Glycerol |
