Experimental procedure
Collection date | 1991-11-01 |
Spacegroup name | P 63 |
Unit cell lengths | 66.900, 66.900, 40.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 1.400 |
R-factor | 0.186 |
Rwork | 0.186 |
R-free | 0.22600 |
RMSD bond length | 0.012 |
RMSD bond angle | 25.400 * |
Data reduction software | XENGEN |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 9999.000 * | |
High resolution limit [Å] | 1.400 | 1.400 * |
Rmerge | 0.040 | 0.207 * |
Number of reflections | 19756 | 3449 * |
Completeness [%] | 92.7 | |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 * | used as seeds * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | 3.2 (M) | ||
2 | 1 | reservoir | sodium phosphate | 20 (mM) | |
3 | 1 | drop | protein | 15 (mg/ml) | |
4 | 1 | drop | 2.5 (M) | ||
5 | 1 | drop | sodium phosphate | 20 (mM) |