2PHP
Crystal structure of the C-terminal domain of protein MJ0236 (Y236_METJA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-26 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.077, 112.141, 67.253 |
| Unit cell angles | 90.00, 109.28, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.030 |
| R-factor | 0.2162 |
| Rwork | 0.216 |
| R-free | 0.23800 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.100 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.084 | 0.413 |
| Number of reflections | 63779 | |
| <I/σ(I)> | 12.8 | |
| Completeness [%] | 97.2 | 83.3 |
| Redundancy | 6.9 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 10% PEG6000, 2.0M Sodium chloride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
