2PHM
STRUCTURE OF PHENYLALANINE HYDROXYLASE DEPHOSPHORYLATED
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1996-05 |
| Detector | WEISSENBERG |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 56.433, 56.433, 299.589 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.000 - 2.600 |
| Rwork | 0.208 |
| R-free | 0.28100 |
| Structure solution method | MIRAS |
| Starting model (for MR) | 1-H |
| RMSD bond length | 0.005 |
| RMSD bond angle | 24.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.101 | 0.450 |
| Total number of observations | 105530 * | |
| Number of reflections | 15307 | |
| <I/σ(I)> | 10.9 | 1.9 |
| Completeness [%] | 95.0 | 95 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 * | 22 * | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.85 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 20 (%) | |
| 3 | 1 | reservoir | sodium citrate | 100 (mM) | |
| 4 | 1 | reservoir | t-butanol | 0.25 (%) | |
| 5 | 1 | reservoir | free glutathione | 10 (mM) |
