2PHA
Crystal structure of native, unliganded human arginase at 1.90 resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X12B |
Synchrotron site | NSLS |
Beamline | X12B |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00 |
Spacegroup name | P 3 |
Unit cell lengths | 90.770, 90.770, 69.740 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 18.030 - 1.900 |
Rwork | 0.198 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2aeb |
RMSD bond angle | 1.500 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | PHASER |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.300 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.077 | 0.237 |
Number of reflections | 49544 | |
<I/σ(I)> | 11.3 | 2.2 |
Redundancy | 1.9 | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 294 | 18-22% Jeffamine ED-2001, 0.1M Hepes (pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 294K |