2PHA

Crystal structure of native, unliganded human arginase at 1.90 resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12B
Synchrotron site	NSLS
Beamline	X12B
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00
Spacegroup name	P 3
Unit cell lengths	90.770, 90.770, 69.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.030 - 1.900
Rwork	0.198
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2aeb
RMSD bond angle	1.500
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.300	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.077	0.237
Number of reflections	49544
<I/σ(I)>	11.3	2.2
Redundancy	1.9	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	294	18-22% Jeffamine ED-2001, 0.1M Hepes (pH 7.0), VAPOR DIFFUSION, HANGING DROP, temperature 294K