2PGS
Crystal structure of a putative deoxyguanosinetriphosphate triphosphohydrolase from Pseudomonas syringae pv. phaseolicola 1448A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-01 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 103.730, 103.730, 159.120 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.310 - 2.350 |
| R-factor | 0.251 |
| Rwork | 0.251 |
| R-free | 0.27300 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.410 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.084 | 0.402 |
| Number of reflections | 22333 | |
| <I/σ(I)> | 10.4 | 2.3 |
| Completeness [%] | 99.5 | 95.2 |
| Redundancy | 24.5 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | Sodium formate, PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
