2PGG
Crystal Structure of a Birnavirus (IBDV) RNA-dependent RNA Polymerase VP1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-10-15 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 122.103, 122.103, 359.017 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.970 - 2.500 |
R-factor | 0.216 |
Rwork | 0.216 |
R-free | 0.25400 |
Structure solution method | MIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.065 | 0.338 |
Number of reflections | 53819 | |
<I/σ(I)> | 30.7 | 4.53 |
Completeness [%] | 96.4 | 72.7 |
Redundancy | 5.7 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 100mM HEPES, 1.3M sodium malonate, 10mM sodium iodide, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |